Qiaamp dna investigator kit 50 from qiagen sample to insight. Orders 80057296 fax 80075951 technical 800dnaprep 8003627737 france qiagen s. The minelute dna cleanup system is specially designed for fast and easy isolation of dna fragments from pcr reactions, agarose gels, or enzymatic reactions with an extremely small elution volume of only 10 l. Isolate the mutated plasmid from the g mutant cultures using the qiagen qiaprep spin miniprep kit. For dna purification from whole blood, plasma, serum, buffy coat, lymphocytes, dried blood spots qiaamp.
For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. Gel extraction qiagen qiaquick gel extraction kit materials. For isolation of genomic, mitochondrial, bacterial, parasite or viral dna. Qiaamp dna mini and blood mini handbook moodle ufsc moodle. For the isolation of large cosmid and plasmid dna constructs, the qiagen largeconstruct kit is available see ordering information on page 48. Qiaamp dna mini kits contain readytouse proteinase k solution, which is dissolved. Qiaamp dna mini and blood mini handbook moodle ufsc. Should any product fail to perform satisfactorily due to any reason other than misuse, qiagen, the distributor of preanalytix products, will replace it free of charge or. Qproteome mitochondria isolation handbook 042006 5 product use limitations qproteome kits are intended for research use only. Sigmaaldrich provides genomic dna purification kits and reagents for the purification of mammalian genomic dna, plant genomic dna, blood genomic dna, bacterial genomic dna. Fresh and frozen whole blood with common anticoagulants, such as citrate, edta, and heparin, may be processed. For the isolation of large cosmid and plasmid dna constructs, the. Dna ffpe tissue kit qiacube, qiagen, qiasymphony dna mini kit. Use of the isolated mutated plasmid dna some of the plasmid dna will be used in restriction digestion experiment and some will be submitted for sequencing by the sanger dideoxy sequencing method.
If you have any questions or experience any difficulties regarding the qiaamp dna micro kit or qiagen products in general, please do not. Our advanced, highquality products and services ensure success from sample to result. Designed for rapid dna cleanup, with extremely high endconcentration of dna, the minelute system includes. Follow the dna purification from blood or body fluids spin protocol procedure in the qiaamp. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for pcr, southern. Sample preparation using the qiacube follows the same steps as the manual procedure i.
Item qiaamp dna investigator kit 50 company qiagen sample to insight. Qiagen plasmid purification handbook harvard university. Viral rna binds specifically to the qiaamp silica membrane, and pure viral rna is eluted in either water or a buffer provided with the kit. Dna binds specifically to the qiaamp silicagel membrane while contaminants pass through. The qiaamp dna mini kit simplifies isolation of dna from human tissue samples with fast spincolumn or vacuum procedures. The genomic dna purification kits are useful in pcr, restriction endonuclease digestions, cloning, southern blots and sequencing. Dna adsorbs to the dneasy membrane in the presence of high concentrations. Dna purification from blood or body fluids spin protocol. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 150 l. Rnasefree dnase set box, containing rnasefree dnase, buffer rdd, and rnasefree water, should be stored immediately upon receipt at 28c. Mix the solution by pipetting up and down before loading the gel. Qiagen qiaamp dna mini kit, 50 preps, 200l sample, 50 to 200l elution volume, whole blood, tissue, cells sample, silica technology, spin column format, manual processing, 20 min.
Rneasy micro handbook 042003 5 all due care and attention should be exercised in the handling of the products. Qiagen genomic tip kit algimed company, llc belarus. The quality of isolated dna is checked by restriction digestion. Qiagens plasmid prep kits are very good, if somewhat expensive my guess is their sequencing service is the same. The rneasy micro kit is shipped at room temperature 1525c. We recommend all users of qiagen products to adhere to the nih guidelines. The paxgene blood dna kit allows isolation of total dna from 8. A comparison of commerciallyavailable automated and manual. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
If purified dna is to be analyzed on a gel, add 1 volume of loading dye to 5 volumes of purified dna. Qiaamp dna micro handbook 082003 3 contents kit contents 4 storage 4 quality control 4 product use limitations 5 product warranty and satisfaction guarantee 5 technical assistance 5 safety information 6 introduction 7 principle and procedure 7 equipment and reagents to be supplied by user 10 important notes 11 eluting pure dna 11 yield and. Dna isolation with qiagen genomictips this protocol applies to. Isolation of genomic dna from lasermicrodissected tissues 38 cleanup of genomic dna 41. Rneasy mini handbook rneasy mini kit rneasy plant mini kit for total rna isolation from. Following the centrifugation, remove the dneasy mini spin column from the collection tube carefully so the column does not come into contact with the flowthrough, as this will result in carryover of ethanol. The qiaamp dna blood mini kit simplifies isolation of dna from blood and related body fluids with fast spincolumn or vacuum procedures see flowchart procedure.
Should any product fail to perform satisfactorily due to any reason other. The qiaamp dna investigator kit provides purification of genomic dna from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. Gel containing dna to extract gel extraction tips buffer qg 3 volumes gel extract 100 mg 100. Spin tubes at 20,000 x g for 5 minutes in centrifuge. Use of this product signifies the agreement of any purchaser or user of the paxgene blood dna kit to the following terms. Nov 14, 2014 although there are differences in the technologies that commerciallyavailable kits employ to isolate total rna from tissue samples, each uses the same general approach. Qiaamp dna investigator kit 50 56504 from qiagen sample. The remaining components of the rneasy micro kit should be stored dry at room temperature. Lyophilized qiagen protease can be stored at room temperature 1525c for up to 6 months without any decrease in performance. I had a lot of problem with the genotyping pcr these days. Dna extraction qiagen kit tuesday, february 05, 20. I was useing this kit to isolate dna from the tail of mice for genotyping. A variety of microorganisms, including bacterial and fungal spores, have been tested successfully with this kit. Variation in prepcr processing of ffpe samples leads to discrepancies in braf and dna was extracted using the qiaamp dna ffpe tissue kit qiagen.
The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed ce. Qiaamp dna mini kit nucleic acid purification bacterial. Qiagen dneasy dna extraction protocol for bacterial cultures adapted from qiagen dneasy handbook, july, 2006. This kit can be used for both gramnegative and grampositive.
Pdf qiaamp dna micro handbook sample to insight qiagen the qiaamp dna ffpe tissue kit is. Qiagen plasmid purification handbook qiagen plasmid midi, maxi, mega, and giga kits qiafilter plasmid midi, maxi, mega, and giga kits endofree plasmid maxi, mega, and giga kits for purification of ultrapure plasmid dna second edition august 2003. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. The dna binding capacity of qiaquick spin columns is tested by. This kit is designed for the rapid preparation of genomic dna from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol.
The kit can be used to purify nucleic acids from a. Qiagen or bd and are distributed for preanalytix by qiagen. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for pcr. Purified dna is of an excellent yield and quality, and is immediately ready for any downstream application including pcr, qpcr, genotyping. Spin kits is monitored routinely and on a lottolot basis. The magattract powersoil dna kf kit can be used for automated isolation of. Qiagen sample and assay technologies qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Although there are differences in the technologies that commerciallyavailable kits employ to isolate total rna from tissue samples, each uses the same general approach. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed cells from urine.
Qiaquick spin kits are tested by isolation of dna fragments of various sizes from either aqueous solution or agarose gel. This protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. The convenient spincolumn procedure reduces handson preparation time to 20 minutes. The data presented here, including cell lysis, pcrdgge dna profiles and sequence analysis of random 16s rrna gene clones, demonstrated that the qiaamp kit has an efficiency similar to the bead beating method in cell lysis and dna extraction and. Do not add bleach or acidic solutions directly to the sampleprepara tion waste. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Elution volume versus dna concentration and recovery. No mechanical homogenization is necessary as the tissues are lysed enzymatically. The paxgene blood dna kit may be used solely in accordance with the paxgene blood dna kit handbook and for use with components contained in the kit only. Plasmidcosmid copy number plasmid and cosmids vary in copy number, depending on the origin of replication they contain, their size, and the size of insert. Purified dna is of an excellent yield and quality, and is immediately ready for any downstream application including pcr, qpcr, genotyping, sequencing and more. A comparison of commerciallyavailable automated and.
Qiaprep miniprep handbook washington state university. No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic, prognostic, therapeutic, or blood banking. The magattract powersoil dna kf kit can be used for automated isolation of microbial dna. The qiaamp viral rna mini kit simplifies purification of viral rna from cellfree body fluids with fast spincolumn or vacuum procedures. The standard protocol uses 50 l buffer eb for elution, since this combines high yield with high concentration. Forceps are to be sterilized in fine science tools heat block at. Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. Mo bios powersoil dna isolation kit handbook qiagen. The purchaser must determine the suitability of the product for its particular use. Transposon mutagenesis session 3 flashcards quizlet.
Prior to using them for other purposes, the user must validate the system in compliance with the applicable law. Purification requires no phenolchloroform extraction or alcohol precipitation, and involves very little handling. Qiaamp dna mini kit and qiaamp dna blood mini kit 092001 7 product use limitations qiaamp kits are intended as generalpurpose devices. Qiaamp dna mini kit bacterial or genomic dna purification from water, filters, or surface swabs by qiagen. These products are not intended for the diagnosis, prevention, or treatment of a disease. Dna purification from dried blood spots qiaamp dna mini kit.
700 1204 865 1223 211 1326 1440 109 814 554 475 452 333 803 720 318 351 1607 523 974 1312 1334 1624 1503 889 243 1535 820 613 10 1426 795 1004 737 1173 942 1607 16 251 84 1285 83 35 608 1246 1227 924 1363 566 369 591